FastQCFastQC Report
Thu 26 May 2016
ERR1356852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063623
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA793667.461854435265127No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA637125.9900923541518No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT437544.11367561626629No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG312172.93496849917687No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA301052.8304201770740196No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT240802.2639600685581263No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC228192.1454030234396964No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG155951.4662150028722585No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG126451.1888610908188335No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG121281.1402536425030296No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG111001.0436028555230565No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT108311.018311939474795No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA102310.9619009743113867No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG99530.935763893785674No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT95010.893267633362573No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG94530.8887547561495004No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA78410.7371972964104763No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA75040.7055131376436952No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC69220.650794501435189No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC59660.5609130302748248No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT50150.47150165049082243No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC46010.4325780845280706No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT45250.4254326956073722No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC42520.39976570645802134No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT38690.36375670702871227No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA38600.36291054255126115No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT38230.35943186636618424No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACCTGTCTCTTATACACAT36160.33997008338480833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.3257733238186839No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCCTGTCTCTTATACACAT33240.3125167470052829No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33000.3102603083987466No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG32520.3057474311856739No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC30940.29089254369264295No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT30060.2826189354686764No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCCTGTCTCTTATACAC29680.2790462410083272No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCCTGTCTCTTATACAC29300.275473546547978No Hit
TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA28910.27180683381235643No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGCTGTCTCTTATACAC28540.2683281576272796No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACCTGTCTCTTATACAC28080.2640033169647516No Hit
GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA28020.26343920731311754No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACCTGTCTCTTATACACATC27160.25535363563969565No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT26930.25319121530843164No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA26900.2529091604826146No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCTGTCTCTTATACACATC26590.24999459394917184No Hit
CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT25530.24002865677030302No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG24960.23466961507977918No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24270.22818235408598722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.22385751342345928No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCTGTCTCTTATACACA22540.2119171924638711No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGCTGTCTCTTATAC22470.21125906453696466No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA22360.21022486350896888No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACCTG22050.2073102969755261No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCCTGTCTCTTATACACA21870.20561796802062385No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCACTGTCTCTTATACACA21860.2055239497453515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.2023273283860917No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCCTG21310.2003529446053724No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCCTGTCTCTTATAC21200.19931874357737656No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21110.19847257909992544No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20820.19574604911702737No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACCTGTCTCTTATACACA20360.1914212084544994No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC19190.18042107024763473No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCACTGTCTCTTATACA18180.17092522444512764No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTGACGCTGCC17340.16302768932225045No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG16870.15860883038445012No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACCTGTCTCTTATACACATCTGACGCTGCC16780.157762665906999No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.1573865928059096No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.15136942318847937No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCCTGTCTCTTATACA15870.1492070028572154No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTGACGCTG15780.14836083837976424No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCC15700.1476086921775855No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCCTGTCTCTTATACACATCTGACGCTG15540.14610439977322792No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCC15380.14460010736887036No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG14820.1393350839536189No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14650.137736773273989No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.13284782295982694No Hit
TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA13360.12560841576385617No Hit
CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG13040.12259983095514106No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC13010.12231777612932403No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12590.11836900856788543No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12540.11789891719152369No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.11667667961298316No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA12100.11376211307954039No Hit
CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA11980.11263389377627223No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11730.11028343689446354No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11370.10689877898465905No Hit
TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG10940.1028559931479481No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10730.10088160936722881No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTGT150.002355784468.9978865
ATCTAAG200.00733653451.789821
CTTAGGT200.00733653451.789822
TTACAGA200.00733653451.789821
TAGACGT200.007340631451.782515
GGCGTCA200.00735703751.7532847
GCGATGT200.00735977451.74841369
GCGAGGG200.00735977451.74841369
ACGTTGT200.00736114351.745988
GCTTATG3100.046.7779051
AAAAGTA2150.043.358922
TTATGTA3300.042.89463
TATGTAC3400.041.632994
AAAAAGT2250.041.4318541
CTTATGT3550.039.8757322
TAGGTAC350.001342995939.4570544
ACAGATG350.001342995939.4570543
GCTTGGT350.001348287139.4255115
TATGGGA655.41997E-737.1806874
ATGGGTG400.00258826434.521675
Produced by FastQC (version 0.11.5)