FastQCFastQC Report
Thu 26 May 2016
ERR1356850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721347
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG40590.23580370488925245No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG36390.21140420844838376No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT27020.1569700937695886No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT26070.15145116005082065No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA25980.15092831369851634No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC23450.1362305217948502No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC22800.13245440925043003No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22090.12832973247114032No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT21950.1275164159231114No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG20160.11711758291616972No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCT18170.10555686912632956No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAAT18100.10515021085231507No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT18100.10515021085231507No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA17740.10305882544309776No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCG150.002357170568.9894840
GTAGCGC309.129888E-657.4979133
GCGCCCT252.5691107E-455.19318811
ACGTATC252.5694794E-455.19158626
GGCTCGG200.00736408851.7421141
CACGTTA306.3026225E-446.01437460
CGTTATC350.001346897639.43516562
TTCGGTC1450.035.6956249
ATCGTCA7650.034.72120710
GCGCGAG400.002598263834.49574311
AACGCGT501.8631533E-434.49574318
GCGATAC400.002598263834.4957439
GCTCGAC400.00259863434.4947433
TCGACTC400.00259863434.4947435
CAATGCG8000.034.06454519
CGACGAG8000.033.63237424
TGGTCGT9700.033.0828551
GCTCGAT9700.032.3695164
CTGGTCG10250.032.31759650
GCTCGTC10850.031.47446439
Produced by FastQC (version 0.11.5)