FastQCFastQC Report
Thu 26 May 2016
ERR1356849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731113
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG32980.1905132709418738No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG32620.1884336839940547No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT24430.1411230809311697No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG22790.1316494070577715No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT22230.12841449402783064No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT21700.12535287991020805No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA21580.12465968426093502No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC19500.11264429300686898No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC19350.11177779844527769No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA18400.10628999955519945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG200.007358427651.75218623
ATGTCCG400.002597629834.4974765
GGAGTCG400.002599102734.493488
ACGCAAG2350.033.759588
CAATGCG6300.032.85853619
GAGTCCT6600.031.88402728
AGTCTCC450.004627604530.6608738
CGACGAG7000.030.55843524
TTGTGTA2750.030.10688269
GCATATA1501.8189894E-1229.9254821
ATCGTCA7150.029.91216510
TCTCTGC5800.029.7357648
GGGTAAA3000.028.7745021
ATGTGCG605.4256455E-428.7478925
TCGCTGG1103.670175E-828.22357412
GTCATCC7850.027.68426713
GTACCCA1254.116373E-927.6235221
TCAAGAC2500.027.6227233
TGTCCGG500.007738749527.59797966
CCGATGT500.007739840527.59717862
Produced by FastQC (version 0.11.5)