FastQCFastQC Report
Thu 26 May 2016
ERR1356849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731113
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG35080.20264419480415202No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG33130.1913797655034651No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG25560.14765067329515752No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT23170.1338445266138028No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT22580.13043631467154368No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT22290.12876109185246717No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA21470.12402425491576806No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC21160.12223349948847938No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC20380.11772772776820462No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18400.10628999955519945RNA PCR Primer, Index 27 (96% over 30bp)
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG18100.10455701043201686No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA17670.10207305935545513No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG17440.10074443436101513No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT17400.1005133691445908No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG17400.1005133691445908No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTC252.5692122E-455.192777
GCGAACG200.00735338751.76115864
CAGCGTT306.3074345E-446.00726345
TCGGTCA306.31642E-445.9939738
GTTCTAA758.62201E-1041.4544451
AGTGGAC350.001348875539.4234114
CGGCGAT451.0027194E-438.327232
CTCCTGA451.0027194E-438.327224
CGACGAG6750.037.81617424
CCGATCA655.460188E-737.14790732
CATCGGT655.460188E-737.14790725
GAGTCCT6950.036.7279428
CAATGCG7100.035.4682119
GTACACG400.002580014834.5453721
CGCTCCA400.002592482134.5114354
TGCGTAC400.002592482134.5114360
CGAACTT501.8591339E-434.50843463
CGGCGGA400.002594687334.50544445
GGCGGAT400.002594687334.50544446
TGAGTAC501.8610289E-434.50245769
Produced by FastQC (version 0.11.5)