FastQCFastQC Report
Thu 26 May 2016
ERR1356844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836659
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGA451.861401E-645.997817
TTGCGCA605.42626E-428.74519335
GAGTACT21850.021.78843512
AGTACTT22550.021.11207613
GTACTTT23350.020.09326214
CAATGCG900.005734716519.16575419
ATATCGT950.007834868518.1570286
TACTTTT25750.018.08651515
TACACCG1150.001107565218.00565
ACTTTTT26850.017.60251216
GTATCAA48700.017.2970051
GTCCTAC2057.693652E-716.8405481
GTCCAAG3700.016.7950331
GTGTTAC1653.817583E-516.7384831
GTACATG33650.016.6203421
GTCTAAT1250.001931346316.5710981
TATACGG1250.001931346316.5710982
CGTGCGC1250.00194063516.5592110
ACATGGG32500.016.356693
TACATGG33850.016.2161792
Produced by FastQC (version 0.11.5)