FastQCFastQC Report
Thu 26 May 2016
ERR1356844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836659
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG400.00259741134.49599532
CGTTCGG400.00259741134.49599535
CGCATAC450.00462460530.66310937
TCGCGTT500.00773585927.5984469
GCGTTCG500.007738115727.59679834
GCGTATC800.002862712821.609071
TATACTG2352.0008883E-1120.555725
GAGTACT21950.019.01601612
GTATTAG2053.725654E-818.5521771
AGTACTT22700.018.2357713
GGTTGCG950.00783796218.15578730
TACCTGG5550.018.0281682
TACGACC1150.001109408818.0011394
GTATATG3103.6379788E-1217.844911
GTATAAG3103.6379788E-1217.844911
GTACATG30500.017.4572941
GTACTTT23750.017.2843114
ACGACCA1200.00147510717.2521235
TCTATAC1402.0807184E-417.2510913
TACATGG31000.016.9171982
Produced by FastQC (version 0.11.5)