FastQCFastQC Report
Thu 26 May 2016
ERR1356842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890402
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG1750.029.57099322
GGTATCA16800.028.7641241
CGACGAG1800.028.7495824
CGTTGCC605.421695E-428.74957859
CGCACTA1750.027.59959458
CTCGATA2050.026.92643565
GTACTAG2950.026.9115161
CAATGCG2100.026.28385519
GTATCAA27150.025.6811161
CGATAAT2200.025.0905467
TGGTCGT2250.024.5357351
TCGATAA2250.024.53297466
ATGCGAC2150.024.06941421
AATGCGA2350.023.48901720
TCGTCTA2250.023.00224754
ATCGTCA2250.022.99966210
ACGAGTC2250.022.99966226
CGCCTAG1202.4583169E-622.9983744
GGGTTCG750.001995446622.99449533
CGTCATC2700.021.72190312
Produced by FastQC (version 0.11.5)