FastQCFastQC Report
Thu 26 May 2016
ERR1356842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890402
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG200.007355412551.75547848
CTCGATA1653.6379788E-1227.18622265
GGTATCA15100.026.7707041
GTATCAA24600.024.9997581
TCGTCGA851.440808E-424.35825562
GTCGTCT1851.8189894E-1124.24036453
CGACGAG1301.8485298E-723.88177724
TCGTCTA1902.5465852E-1123.61174254
CATGGGT2950.023.387054
CGATAAT1953.8198777E-1123.00243467
CAATGCG1854.838512E-1022.3757219
TGACGCG1103.2688426E-521.95193732
CGTGCGT800.002900923821.55993812
ATCAACG27900.021.5135733
TGCGACG1455.2962605E-721.4112522
CAACGCA28900.021.0078855
TGCGATC1154.605353E-520.99750534
GCTCGAT2151.382432E-1020.86384464
GTACTAG3000.020.7301351
TCAACGC29000.020.6975424
Produced by FastQC (version 0.11.5)