FastQCFastQC Report
Thu 26 May 2016
ERR1356837_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356837_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244001
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG14880.11961405175719311No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC200.007359495451.74942823
GTAGCGC352.265582E-549.312933
CGACGAG7250.045.20639424
CAATGCG7500.043.2395219
ATCGTCA8100.041.738610
CTGGTCG9200.040.13585350
GCGCGAG350.001348200439.4265511
CGCGAGA350.001348466839.42496512
GAGTCCT8600.039.31193528
TGGTCGT9400.039.28190251
GTCATCC8550.039.13519313
CGATATA901.2369128E-1038.32828562
CCAATGC8750.037.8510118
AGTCCTA8850.037.810129
GCTCGAT9800.036.9594264
GTCAGCG10350.035.99670843
GTCTACC10500.035.82397556
GTCCTAC9400.035.5978130
GTCGTCT10600.034.83489253
TCGTCTA10750.034.6698454
Produced by FastQC (version 0.11.5)