FastQCFastQC Report
Thu 26 May 2016
ERR1356837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244001
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAG200.007308415651.8406641
CACGTCG200.007360921251.74689517
ATCGTCA8550.044.78318410
CGACGAG8500.044.6407924
CAATGCG8650.044.26902419
AGTCCTA9150.042.22358329
GTCATCC9700.040.18831313
CCAATGC9900.040.07335318
TTACGTG350.001348791139.42303526
GAGTCCT9700.039.1182228
GCTCGAT8650.037.88942364
TGGTCGT8800.037.6416751
CTGGTCG8850.037.4290150
GTCAGCG9400.036.3343
TATGCCG3150.036.13778343
CTCGATA9150.035.440565
CATCCAA11200.034.80594615
CTAGACG709.732466E-734.502094
TACCCCG501.860844E-434.502095
TCATCCA11300.034.4979314
Produced by FastQC (version 0.11.5)