FastQCFastQC Report
Thu 26 May 2016
ERR1356832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159973
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.11345091652995372No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12330.1062955775694779No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG6050.041.05633519
CGACGAG5800.041.04335824
ATCGTCA5900.040.93069510
GTCAGCG7100.040.81363343
TCGGCGC350.001348639139.42371739
TGGTCGT7400.038.23491751
GTCGTCT7700.037.1933653
CTGGTCG7700.036.74524750
GCTCGAT7950.035.58205864
GCGCTGG8150.035.5600447
CTCGATA8100.035.35054865
TGCGACG6900.035.00021722
GTCATCC7050.034.741913
GTCTACC8200.034.08389356
TCGTCTA8500.033.69280654
GTACTAG8150.033.460691
CGCCAGC8700.033.30193334
CGATAAT8500.032.8738467
TCGATAA8550.032.68300666
GCTCGTC8550.032.2767339
Produced by FastQC (version 0.11.5)