FastQCFastQC Report
Thu 26 May 2016
ERR1356832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159973
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.1072438755039988No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG200.007350072751.76597655
GTACGTC200.00736386951.7414329
ATCGTCA6450.039.57484410
ACACGCG350.001348920939.42204352
CTCGACT754.026697E-836.79390727
CGACGAG6850.036.76033824
TGGTCGT8150.036.41457451
CAATGCG7050.036.20989219
GCTCGAT8100.036.20863764
GTCAGCG8350.035.52705443
GGGCGGC400.002592965334.50916346
GCTGCGC807.104791E-834.4942932
GTCGTCT8650.034.294953
CTGGTCG8800.034.11701250
GTACTAG9100.033.0464321
GTCTACC9400.032.30784656
GTCATCC8050.032.1403113
TCGTCTA9250.032.0855854
TCGATAA9200.031.87659366
TGCGACG8000.031.47603822
Produced by FastQC (version 0.11.5)