FastQCFastQC Report
Thu 26 May 2016
ERR1356828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335502
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG3400.028.41067122
GGTATCA40700.027.5663851
CGACGAG3500.026.61326424
ATGCGAC3650.026.46473321
GTATCAA64550.025.8845081
CGCACTA3500.025.62868358
TACGTGT4800.022.99911569
CAATGCG3950.022.70701419
GTACATG80200.021.7804571
TTACGTG5550.021.75591968
CTCGATA3350.021.62649765
ACGTCGT800.002900965521.5616723
TACATGG80900.021.4639842
AATGCGA4800.020.84294720
CGTACGA2650.020.83027860
GTACTAG6800.020.8144911
ACATGGG80800.020.7215023
ACCGTCG2501.8189894E-1220.69920323
TCAACGC78300.020.6776854
CAACGCA78300.020.6309415
Produced by FastQC (version 0.11.5)