FastQCFastQC Report
Thu 26 May 2016
ERR1356828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335502
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39400.029.554071
CTCGATA3800.026.32861165
GCTCGAT4050.025.5574264
GTATCAA63150.024.9502621
CGATAAT4250.023.54087667
GTACATG76050.020.8998051
TCGTCTA4650.020.78149454
CGTACGA3000.020.70727360
CGTCATT5000.020.69707726
CTCGTAT5200.020.56440539
TACATGG76000.020.5172822
ACATGGG74650.020.2875543
TATGCCG5300.020.17639543
ATCAACG76750.019.9122493
TAGGTCG5200.019.90103721
TATCAAC78400.019.8011952
CAACGCA78350.019.5940975
TCAACGC78500.019.5122934
ACCGTCG3550.019.43387623
CATTGCG5200.019.23766929
Produced by FastQC (version 0.11.5)