FastQCFastQC Report
Thu 26 May 2016
ERR1356827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471602
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT4350.030.13450267
CTCGATA4700.029.35836265
GTATCAA56800.027.7795051
GCTCGAT5250.027.59629864
GGTATCA35850.026.9665571
CGACGAG3650.025.5207424
TCGATAA5500.025.08805366
TGCGACG3550.024.29596122
ATCAACG64400.023.4820543
CAACGCA64950.023.2789635
TTACGTG4900.023.2325269
TGGTCGT5800.023.2031551
TATCAAC67350.022.9666182
GCTCGTC6250.022.6280539
AACGCAG68050.022.5091786
TCAACGC68150.022.3419254
CAATGCG4200.022.1787419
GAGTACT40050.021.01718512
CGTCTAC6700.020.6005155
CATTGCG6950.020.35061329
Produced by FastQC (version 0.11.5)