FastQCFastQC Report
Thu 26 May 2016
ERR1356827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471602
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATA4600.030.00681165
GCTCGAT4800.029.4772364
CGACGAG3850.028.6690724
CGATAAT5000.027.60570567
TGCGACG3900.027.41709522
GGTATCA36450.026.6383291
CAATGCG4300.026.47204219
GTATCAA55000.023.6851881
TGGTCGT6300.023.0066251
TATGCCG5700.022.99498443
ATGCGAC5600.022.1746321
TCGATAA6300.021.90929266
GCTCGTC6300.021.89998439
CGTCTAC6600.021.44145855
CATTGCG6750.020.95098529
GTCGTCT7000.020.69548453
CGTCATT6850.020.6451326
ATCGTCA5250.020.36698310
GTACTAG7300.020.3537221
ATCAACG62450.020.2174193
Produced by FastQC (version 0.11.5)