FastQCFastQC Report
Thu 26 May 2016
ERR1356820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420620
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42010.29571595500556097No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33040.23257450972110771No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30370.2137798989173741No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24000.1689403218313131No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG22610.15915586152524952No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG20090.14141712773296167No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG17830.12550858076051302No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA17510.12325604313609552No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG16790.11818783348115612No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT16320.11487941884529289No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT16210.11410510903689938No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC15450.1087553321789078No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA15300.10769945516746209No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT15100.10629161915220114No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA14920.10502456673846629No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT150.002354502269.0085655
CGTATCG200.007355778551.7564254
TACGTTC200.007355778551.7564251
GTGCCGT200.00736090251.7473076
GCACATC451.8614792E-646.00084723
AGGTTAG350.0013475739.43068347
GATAGTC451.0028233E-438.3259440
ATACAGC557.401841E-637.63175642
GAGTCCT4200.036.9623628
ATCGTCA4350.036.4808610
CGACGAG4350.035.6903124
GCATATA1102.7284841E-1134.5249561
GATATAC501.8622089E-434.49820363
CAATGCG4650.033.38653619
CTCACCT553.255041E-431.36752955
CGACCCC553.2584046E-431.36200568
ACAGCGT553.259751E-431.35979544
GTCAGCG4850.031.29513743
TCAAGAC1550.031.1827343
TAGTGCC450.00460752430.6877654
Produced by FastQC (version 0.11.5)