FastQCFastQC Report
Thu 26 May 2016
ERR1356820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420620
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41580.29268910757274996No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32230.22687277385930088No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA30210.21265363010516536No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23530.16563190719544987No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG23040.16218270895806056No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG21670.1525390322535231No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG19090.13437794765665695No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT18240.12839464459179795No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA17210.12114428911320409No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG16340.115020202446819No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA15730.11072630260027312No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15690.11044473539722094No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT15320.1078402387689882No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC15230.10720671256212076No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT15230.10720671256212076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGCG301.1334487E-768.985514
GTACGCA309.138486E-657.48791511
GGGTCGC200.00736550751.7391246
CGGGGTG306.3086435E-446.00490666
TCCCCGC306.3130236E-445.9984369
CATCGGT405.0098726E-543.11441825
GCTGTAT405.0098726E-543.11441829
GTATCGA350.00133814939.4871
TCATTGT350.001349318939.42028413
GGACAGA451.0029254E-438.325286
TCGCCGC451.0031333E-438.3239340
CGGTCCG451.0031333E-438.3239328
ATCGGTC451.0031333E-438.3239326
TTCGGTC807.072231E-834.513449
CGGTCCA807.072231E-834.513451
TCCGGAT400.00259832134.4951818
CAATGCG4000.033.63279719
CGACGAG3950.033.18173224
ATCGTCA4200.032.0289810
GACAGAT553.2614294E-431.3570447
Produced by FastQC (version 0.11.5)