FastQCFastQC Report
Thu 26 May 2016
ERR1356818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641867
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.17784631763717768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.12126438986836327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.10183528872923324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG5200.039.14276519
ATCGTCA5550.038.53674710
GCTCGAT6900.038.4962164
GTCAGCG7400.037.75979643
TCGTCTA7250.037.1260354
TGGTCGT7400.036.83979851
CTCGATA7250.036.6377765
CTGGTCG7650.035.63588750
GTCGTCT7650.035.63588753
GCAACGA400.002597911334.4966058
TCGATAA7700.034.49660566
CGATAAT7800.034.0543467
CGACGAG6000.033.92372524
GTCTACC8100.033.65611656
GCTCGTC8500.033.27704639
AGTCCTA6350.032.05196429
GCGCTGG8800.031.3624647
CGCCAGC9800.029.2138434
TACCCCG953.1900527E-729.0683695
GTCATCC7400.028.4363913
Produced by FastQC (version 0.11.5)