FastQCFastQC Report
Thu 26 May 2016
ERR1356818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641867
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.16743134492623338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.11815816993702902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.10896132268935303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG451.0026177E-438.32771741
GCTCGTC7350.035.66824339
CTGGTCG7400.034.97280550
TGGTCGT7400.034.97280551
ATCGTCA5750.034.7959610
CAATGCG5800.033.90123719
TCGTCTA7700.033.61227854
GTCGTCT7950.033.410253
GCTCGAT7500.033.12422664
GTCAGCG8100.031.93976643
GTCTACC8350.031.40902956
CGACGAG6300.031.20971124
CGCCAGC8350.030.98348434
GTCGTCA8550.030.66217224
ATGCCGC450.004626577730.66217244
CCACGTA450.004626577730.66217232
CGTCATT8600.030.48390426
TCGATAA8150.030.48241866
AGGTCGT8550.030.25872222
CTCGATA8350.029.752365
Produced by FastQC (version 0.11.5)