FastQCFastQC Report
Thu 26 May 2016
ERR1356812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951096
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.1262879940300221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCA7650.035.6230710
CAATGCG8400.035.3216119
ATTCGCT958.813004E-932.6801910
CGACGAG9000.031.05095524
GCTCGTC9400.030.82441339
GCGCGTT450.00461813730.67390652
GTCATCC9150.030.53722813
GCTCGAT9200.030.37204664
TGGTCGT9100.029.19919851
CTGGTCG9500.028.33300250
GTACTAG11100.028.304981
TGCGACG10000.028.29087322
CGTCATT10250.028.26969326
CTCGATA9800.028.16052265
CGTAGCG756.173005E-527.59518836
CGCCAGC11450.027.41092134
GTCAGCG10250.027.26006143
CATTGCG10650.027.2079229
CGATAAT9900.027.1791767
CCAATGC10900.026.58729618
Produced by FastQC (version 0.11.5)