FastQCFastQC Report
Thu 26 May 2016
ERR1356812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951096
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23630.12111141635265511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT11200.037.58711651
CGACGAG8300.037.403924
GCTCGTC12150.036.056239
CAATGCG9200.035.9963219
ATCGTCA8850.035.85890610
CGTCATT12650.034.6310526
CTGGTCG12300.034.50620350
ATCGCCG1003.8198777E-1034.4947139
GCTCGAT11650.034.35634664
GTCGTCA13350.033.5903524
GTCAGCG12950.033.5624243
CAGTACG1350.033.2171259
GCGCTGG12900.032.90126447
CGCCAGC13900.032.75756534
GTCGTCT12850.032.7498453
CATTGCG13600.032.46560729
GTCTACC13000.032.38855756
TCGTCTA13100.032.1413254
TCAGCGC13800.031.7451344
CTCGATA12750.031.39146465
Produced by FastQC (version 0.11.5)