FastQCFastQC Report
Thu 26 May 2016
ERR1356811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877799
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC200.007364002551.74244730
CAATGCG8750.041.7915319
ATCGTCA9000.039.4807310
CGACGAG9500.037.40379324
GTCATCC9650.036.46294413
AGTCCTA9950.034.6683129
ACGGCGA601.3411574E-534.50691651
GCGCGAG400.002597592834.49772311
CTTCGAA400.002597932234.49680346
CGCAGCG905.1986717E-934.49680314
GAGTCCT10200.033.481328
GCTCGTC12300.033.3722939
TGGTCGT11400.033.29614651
GCGCTGG12350.031.84744547
CTGGTCG11950.031.47492650
CGCGAGA553.2590682E-431.36156512
GTCAGCG12650.031.3607343
TGCGACG11500.031.1987722
GTACGCA1001.4519173E-831.0479511
ATCGCCG1001.4533725E-831.04464539
Produced by FastQC (version 0.11.5)