FastQCFastQC Report
Thu 26 May 2016
ERR1356811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1877799
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGT10750.038.8377351
CTGGTCG11250.037.41831250
ATCGTCA8250.036.7960810
GCGCTGG12200.035.9187247
CGACGAG8650.035.8911824
CAATGCG8700.035.68680219
GTCAGCG12150.034.92127643
GCTCGTC12350.034.63506339
CGCGTTG709.748728E-734.49632610
GCTCGAT12000.034.21523364
GAACCGT1800.032.579866
GTCATCC9650.032.53021213
GTGCGTT851.207718E-732.47491550
GTCTACC13400.031.41714156
TCAGCGC13800.031.2458444
CGCCAGC14250.031.2274234
GTCGTCT13550.030.80401853
TCGATAA13350.030.75444866
GTAATCG450.004626442730.66258223
GTACTAG11550.030.2187271
Produced by FastQC (version 0.11.5)