FastQCFastQC Report
Thu 26 May 2016
ERR1356808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261280
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG8150.035.9823819
TGGTCGT10550.034.3463651
GCTCGAT10550.034.00420464
ATCGTCA8600.033.69542710
CTCGATA10800.033.5386965
GTCGTCT10950.033.40685753
GTCAGCG11450.032.53787643
CTGGTCG11050.031.85530550
GCTCGTC11700.030.95399539
CACGCGG450.004629140330.65919739
TCGTCTA12250.030.1400254
GTCTACC12450.029.93299956
CGATAAT12200.029.68998567
CGTCTAC12750.029.22869355
CGTCATT13500.028.36289426
GCGCTGG12700.028.25383647
CGCCAGC13100.028.17252334
TATTCGC756.1689076E-527.598169
GTCGTCA14000.027.35416624
TGCGACG10600.027.3402222
Produced by FastQC (version 0.11.5)