FastQCFastQC Report
Thu 26 May 2016
ERR1356808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261280
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG8750.042.97009319
CGACGAG8750.042.5758724
ATCGTCA9000.042.15974810
GTACCGT501.863783E-434.494346
GCTCGAT10450.033.0191664
GTCGTCT10350.032.99458753
TGCGACG11350.032.51889422
TGGTCGT10800.031.9540451
GTACTAG12700.031.8423211
CTCGATA11050.031.2228165
CTGGTCG11300.030.84554950
AGTCCTA12100.030.78833629
ACGAGTC12250.030.41133526
GTCAGCG11700.030.07198743
GCTCGTC11850.029.9824239
GTCTACC11750.029.66422856
GAGTCCT12650.029.44971328
CGCCAGC12350.029.32717134
GCGCTGG12150.028.97166447
CGTCATC14100.028.86760512
Produced by FastQC (version 0.11.5)