FastQCFastQC Report
Thu 26 May 2016
ERR1356805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703090
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA76320.44812664040068345No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG306.309084E-446.00477620
CGAAGTA350.001348674939.4245630
ATCGTCA5900.035.6669110
CGTTGTC400.00259760634.497510
TGGTCGT6850.033.75251
GTCTACC7000.032.53386356
GTCAGCG7750.032.4925143
CAATGCG6400.032.3452119
GCATATA1305.456968E-1231.8728771
GCTCGAT6850.031.72669864
CATTGCG8200.031.55166629
GCTCGTC8100.031.50975639
CGCCAGC8450.031.42359234
GTCGTCT7500.030.82682653
CTCGATA7200.029.7053165
CGTCATT8500.029.62726626
GAGTCCT7100.029.1519628
ATGCGTT605.426513E-428.74707427
TCGATAA7450.028.70848766
GTCGTCA8800.028.62229224
Produced by FastQC (version 0.11.5)