FastQCFastQC Report
Thu 26 May 2016
ERR1356805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703090
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC200.00736466651.74106211
CACGTAT200.00736466651.74106252
CGACGAG5700.036.30951724
TACCCCG1250.035.8780145
CAATGCG5800.035.68453619
GTCTACC5700.033.90978656
ATCGTCA6450.032.6222710
AAACGTA553.2525705E-431.37203646
GTCAGCG6400.031.26022543
TGGTCGT6250.030.36812851
GCTCGAT6150.030.29634564
GTCGTCT6300.030.11384653
TCGATAA6600.028.75178566
CTCGATA6600.028.75178565
GAGTCCT7350.028.62770728
GCTCGTC7150.028.46361439
TATGCCG14600.028.11500743
CTCGTAT15050.027.96194639
GTCCTAC7600.027.23213630
TCGTATG15550.027.06284740
Produced by FastQC (version 0.11.5)