FastQCFastQC Report
Thu 26 May 2016
ERR1356800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693430
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA70840.41832257607341317No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG40910.2415806971649256No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG40900.2415216454178797No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC35790.21134620267740623No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA29610.17485222300301753No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT29470.17402549854437444No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT28490.16823842733387268No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG28010.16540394347566773No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT27990.16528583998157584No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG27400.1618017869058656No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC24520.14479488375663596No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24360.14385005580390098No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC23640.13959833001659355No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA23400.13818108808749105No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGGCGCTCTAGGCTA21220.12530780723147694No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTG20400.12046556397371017No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT20380.12034746047961829No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCT20230.11946168427392925No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAACCAGATGTGTG19760.11668625216277023No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG19700.11633194168049461No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA19060.1125526298695547No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTG18820.11113538794045222No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCA18540.10948193902316601No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA18290.10800564534701759No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCT17980.10617504118859357No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT17660.1042853852831236No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA17310.10221857413651583No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCA17190.1015099531719646No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC200.007361847451.7460610
TACCCCG350.001345373539.444215
CAATGCG7000.037.9515919
CGACGAG7150.037.15540324
GAGTCCT7600.035.86016528
AGTCCTA7700.035.3944529
ATCGTCA7700.034.9453910
CTACGCT400.002590509634.5167434
GTCATCC7950.034.28040713
TGGTCGT10450.033.34885451
TTCGGTC1056.4210326E-1032.86046249
GTCAGCG10850.032.4307143
GCAAACG553.259129E-431.36124815
GATGGCC553.259129E-431.3612489
ATGTGCC553.2596933E-431.3603237
ACATGTC553.2596933E-431.3603237
CTGGTCG11250.031.28408450
GTCGTCT11200.031.11567153
CGGCGCT450.00462502530.6643310
TTAAGGT1251.2369128E-1030.3747314
Produced by FastQC (version 0.11.5)