FastQCFastQC Report
Thu 26 May 2016
ERR1356794_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356794_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640064
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG459.981775E-538.356615
CAATGCG7000.036.9607919
ATCGTCA7300.034.9692910
CGACGAG6900.034.4967424
CCCGATA400.0025982534.4956813
GTCATCC7550.032.89654513
AGTCCTA8100.029.81199529
GTACTAG9700.029.5457571
GAGTCCT8500.029.22076628
GTCCTAC8350.028.91941830
GTACCCA1254.116373E-927.6235031
TGGTCGT8000.027.60833551
CACTATC9250.027.59570536
CCTACAC9450.026.64502332
CCAATGC9750.026.53595218
CTGGTCG8200.026.51410550
CTACACT9550.026.3668233
TAGGCAT14200.026.255375
CTAGGCA14950.025.1752684
CGTGGTT7150.025.08700436
Produced by FastQC (version 0.11.5)