FastQCFastQC Report
Thu 26 May 2016
ERR1356794_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356794_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640064
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG5500.038.88965619
CGACGAG5350.038.68562324
ATCGTCA5800.036.87362310
CTGGTCG7450.036.12845250
TGGTCGT7350.035.21153351
GTCTACC8050.032.5793256
GTCGTCT8100.031.93951853
GCTCGAT8050.031.28852364
GTCATCC6950.031.27237513
AGTCCTA6650.031.12301829
CGTCACT450.00458412330.7200051
GCCGGAG450.004618474330.6731648
GCTCGTC8750.030.35531839
GTCAGCG8700.030.13328443
CTCGATA8400.029.98117465
CGCCAGC9250.029.46032134
TCGTCTA8800.029.41053454
GAGTCCT7300.028.82432228
GCGCTGG9400.028.6337247
ACCGTCG8900.028.2933923
Produced by FastQC (version 0.11.5)