FastQCFastQC Report
Thu 26 May 2016
ERR1356792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925650
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA63150.3279412146547919No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG44120.2291174408641238No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42100.2186274764365279No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34340.17832939526912991No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC32120.1668008205021681No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA31370.16290603172954587No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT30770.1597902007114481No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG28600.14852127852932775No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT28330.14711915457118377No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC26220.13616181549087322No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG24870.1291511957001532No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG23730.1232311167657674No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23720.1231791862487991No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT23560.12234829797730636No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTT23040.11964791109495496No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21530.1118064030327422No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20320.10552281047957832No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG19770.10266663204632202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA200.007361974651.746112
CGCGGTA350.00134888839.42355343
CAATGCG7050.036.2109819
CGACGAG7200.035.45842724
ATCGTCA7650.034.72287810
CTGGTAC501.8627082E-434.49740266
TCGCTGG1056.4392225E-1032.85466812
CGAAGCT1056.4392225E-1032.85466828
GAGTCCT7900.032.750728
TGGTCGT6650.032.69109351
TCCGCAC652.3095323E-531.8719083
GTCATCC8500.031.65561513
ACCGTGT1101.0604708E-931.3596468
AACCGTG1101.0622898E-931.3588317
GAACCGT1251.2551027E-1030.3569246
TCAAGAC1600.030.2119143
GTCTACC7350.029.57611856
GTACCCA953.183086E-729.0761261
ACGCAAG1900.029.0489358
GTCAGCG7600.029.04893543
Produced by FastQC (version 0.11.5)