FastQCFastQC Report
Thu 26 May 2016
ERR1356790_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356790_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613075
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42880.2658276893510841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.18777800164282504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.15857911132464392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24390.1512018969979697No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA18120.11233203663809804No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC17280.1071245912310339No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.10501681570912699No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGC200.007359632851.7498822
AGTCTAA200.007365049351.74025740
CAATGCG2300.044.99710519
ATCGTCA2400.044.55963510
CGACGAG2550.041.94108624
GAGTCCT2700.039.60856628
CGGTCCA601.34176225E-534.50420451
CGAGTAA400.002597462234.49778411
GAGGCGA400.002597462234.49778427
ATCGGCG400.002599042834.49350441
CGTTAAC1253.6379788E-1233.1466561
TCCGATC553.255279E-431.36745852
GTCAGCG2450.030.9756843
TGGTCGT2700.030.67040451
GTGCGTC901.9971594E-730.66469413
GCACCGC450.00462749930.6608937
TAGGCAT6350.030.4440165
CATATAG4350.028.5747033
GTCATCC3850.027.77743513
ATTCCGA500.007731227727.60336550