FastQCFastQC Report
Thu 26 May 2016
ERR1356790_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356790_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613075
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42080.2608682175348325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.19069169133487282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26670.16533639167428668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23140.14345272228507663No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC18270.11326193760364521No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17250.10693861103792447No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA17010.105450769493049No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTTG200.00736423651.74170325
TCTAGAC1450.040.445553
ACTGACG702.1931555E-839.4222523
CCTAAGC350.001349042639.4222528
CGTCAAC350.001349042639.4222542
CGCTTTA451.0026918E-438.32718726
CGGTCCA557.3855153E-637.6443351
ATCGTCA2650.035.14639310
CGACGAG2750.035.12163524
AACCGTG501.8631667E-434.4955377
CCGTGTT501.8631667E-434.4955379
CACAGTG400.002598291734.4955377
GAACCGT601.3440731E-534.4955376
TCGCTGG807.107337E-834.4944712
ACATACG905.2004907E-934.4944717
TGGTCGT3650.034.034651
GCTCGTC4000.033.63210739
GTCAGCG3600.033.5362943
CAATGCG2950.032.74051319
ATTCGCT851.2103192E-732.4663910