FastQCFastQC Report
Thu 26 May 2016
ERR1356789_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356789_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577211
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGATCG451.8653154E-645.9875736
CGGTCCG856.730261E-1140.58756328
ATCGGTC801.6425474E-938.8118626
CGGTCCA1550.037.84648551
GTGCGTT952.2009772E-1036.32325450
TTCGGTC1650.035.55275749
TGGTCGT7950.032.9879151
CCGATCA958.825737E-932.67434332
TCAAGAC1700.032.493753
CGACGAG6300.032.3110424
GTGTTCA2250.032.199479
CAATGCG6350.032.05255519
GTCTACC8550.031.88070956
GTCAGCG8400.031.61946343
ATCGTCA6650.031.12730610
CATCGGT1001.45082595E-831.04948625
GCTCGTC8650.030.70268439
GCGCTGG8800.030.5829247
GTCATCC6850.030.21752213
TTAAGGT1950.030.0983814
Produced by FastQC (version 0.11.5)