FastQCFastQC Report
Thu 26 May 2016
ERR1356789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577211
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC200.007351437651.76441656
CGCGGTA200.00736342951.74308431
GCTACGT503.847248E-641.39446338
TCCGCAC400.00259550334.5030443
AACCGTG1400.034.4953887
TGGTCGT7650.034.28079651
TCACCGG1850.033.5630826
GTCAGCG8050.033.42410343
GAACCGT1450.033.3058936
ATCCGCC1056.4210326E-1032.86629560
ATCGTCA7000.032.0314310
GCTCGTC8400.032.0314339
CGACGAG7150.031.35944224
GCGCTGG8600.031.29643447
CCGTGTT1550.031.1571259
CAATGCG7100.031.0944319
GACCTGC450.004626232730.6625656
TGAACCG1600.030.1901655
GTCTACC8700.030.14632656
CTGGTCG8700.030.1434650