FastQCFastQC Report
Thu 26 May 2016
ERR1356788_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356788_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858956
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.034.375051
GTATCAA61550.029.1057261
ATCAACG76000.023.298053
TATCAAC76750.023.161572
CAACGCA77300.022.8150845
TCAACGC77850.022.7000664
AACGCAG78800.022.6344386
CGCGTTG800.002901082221.5612463
CGAATAA800.002901535221.5606641
GTACATG84250.021.4274711
TACATGG83300.021.050282
CGTACGA1851.1772499E-820.5139760
ACATGGG82200.020.491033
GTCGGTA2553.6379788E-1220.29238526
TACGGTC1409.355361E-619.71260312
ACGCAGA90000.019.4338637
CGCAGAG90000.019.3188698
TGCGACG2406.0936145E-1018.68741222
ACGGTCG1301.1822979E-418.57533613
GAGTACT52750.018.44202612
Produced by FastQC (version 0.11.5)