FastQCFastQC Report
Thu 26 May 2016
ERR1356788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858956
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.031.9653851
CGATAAT3700.028.90452667
GTATCAA59700.026.4542881
CTCGATA4050.026.40660565
CGACGAG2800.025.8714324
GTCGTCT4700.024.22006253
GCTCGAT4450.024.03685864
TCGTCTA4650.023.74887854
TATGCCG3800.023.60200743
CTCGTAT3700.023.30759439
CTGGTCG4750.023.2451550
GCTCGTC5050.023.22451839
CGTACGA2700.023.00672760
GTCAGCG4850.022.75974743
TGGTCGT4950.022.30595251
CGTCTAC5000.022.08645855
CGGTTAC2057.4578566E-1121.8750328
TATCAAC73000.021.4094072
ATCAACG72750.021.388133
GTACATG85200.021.375941
Produced by FastQC (version 0.11.5)