FastQCFastQC Report
Thu 26 May 2016
ERR1356785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439456
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT4600.023.99822467
ACCGTCG4750.023.9676323
TGCGACG4400.023.5219522
GCTCGAT5100.021.64545664
GTACATG109200.021.5961881
TACATGG109050.021.2459352
CGCGATA2451.8189894E-1221.12088814
CGTACGA4800.020.84263660
CGGGCGT5800.020.8174256
GTATCAA92100.020.8071381
CATTGCG6150.020.75456229
CATGGGG62200.020.3729884
ACATGGG111000.020.3127753
CAATGCG5150.020.095619
GTACTAG7750.020.0488661
CGTCTAC6350.019.5616255
CGACGAG5300.019.52765724
CGTCATT6600.019.33947826
CCGTCGG5900.019.29597524
ATGCGAC5950.019.13382321
Produced by FastQC (version 0.11.5)