FastQCFastQC Report
Thu 26 May 2016
ERR1356785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439456
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT4750.030.50845367
CTCGATA5350.028.37854665
TGGTCGT5200.027.20979151
TGCGACG5150.026.79068222
GCTCGAT5450.026.59484564
TCGTCTA6100.024.89601754
CGCGAAC700.001335854124.63785714
GCTCGTC6100.024.3147439
CGACGAG5950.023.76828424
GTACTAG8150.023.3181111
CTGGTCG6250.022.63854650
CAATGCG6600.022.47363319
GGTATCA65600.022.2804931
TCGATAA6750.021.98052666
CGTCATT7100.021.37594626
ATCGTCA6950.021.3414310
GTCTACC6900.021.00908956
CGTCTAC7100.020.90340855
GTATCAA93000.020.843381
CGCGAAT4150.020.77891535
Produced by FastQC (version 0.11.5)