ERR1356779_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1356779_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1653644
Sequences flagged as poor quality	0
Sequence length	75
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG	3188	0.19278635546707756	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG	3177	0.19212115787920495	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT	2553	0.15438631289443194	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA	2490	0.1505765448911616	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT	2465	0.1490647321914511	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCT	2402	0.14525496418818076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2372	0.14344078894852821	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	2371	0.14338031644053978	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC	2243	0.13563983541802227	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2152	0.1301368371910762	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	2150	0.13001589217509935	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	2001	0.12100548848482504	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	1951	0.1179818630854041	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC	1914	0.11574438028983264	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG	1836	0.11102752466673602	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG	1732	0.10473838383594049	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA	1663	0.10056578078473964	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACTA	15	0.0023548733	69.006325	60
CATCGGT	25	2.569807E-4	55.190033	25
ACCGTTC	20	0.007362216	51.74535	8
CGAGTGA	30	6.315056E-4	45.995865	65
AGATCGA	30	6.316939E-4	45.993084	61
ATCGGTC	30	6.317881E-4	45.99169	26
CTCCGTA	40	4.9967042E-5	43.134174	51
TGCGTAC	50	3.8414037E-6	41.403793	60
GTACCCA	85	6.548362E-11	40.626373	1
CGGCACC	60	2.916422E-7	40.241512	31
CAGATCG	35	0.0013473822	39.432182	60
ACCACAG	35	0.001347783	39.4298	24
TCGAGTG	35	0.0013487856	39.423836	64
TCACAAG	35	0.0013489863	39.42264	14
ACGACGT	55	7.391709E-6	37.639812	59
CGTAAGG	55	7.4024574E-6	37.631844	63
GCGGCAC	55	7.4055315E-6	37.629566	30
GGGAGTG	55	7.4086056E-6	37.627293	39
CGGCGAT	55	7.4132186E-6	37.62388	32
ATCGTCA	485	0.0	34.852535	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content