FastQCFastQC Report
Thu 26 May 2016
ERR1356777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746619
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG7700.039.4291819
ATGCGTA1050.039.4257913
TGCGTAG1050.039.4257914
ATCGTCA7950.038.62102510
GAGTCCT8650.034.69697628
ATCGCCG807.112976E-834.4916439
GTCATCC8950.034.30484413
AATGCGT1253.6379788E-1233.11766412
CGGTCCG1154.7293724E-1132.99767328
ATCGGTC1207.8216544E-1131.6227726
CTTCGTA450.00462493730.66450127
TACTTCG450.00462493730.66450125
CGCAGCG901.9974323E-730.66450114
CCAATGC10300.030.14609318
TCCGATC1501.8189894E-1229.89703431
TACCCCG953.1897434E-729.0688975
TCGCCGC953.2114076E-729.04642340
GTCCTAC10550.028.44741630
TGGTCGT8100.028.1171851
TCATCCA11150.027.84556814
Produced by FastQC (version 0.11.5)