FastQCFastQC Report
Thu 26 May 2016
ERR1356777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1356777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746619
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC306.3163106E-445.994169
TCGCCGC1400.036.95853440
CGACACT1151.8189894E-1235.994414
CGCAGCG1350.035.7722114
ATCGCCG1350.035.7722139
CGCGGGC400.002592488434.5114360
CGACGAG7100.034.49463324
TCGTGCG400.002598684534.49463314
CAATGCG7350.032.85202819
TGGTCGT7900.032.7602651
TTCGGTC851.2067358E-732.47762349
CGGTCCA851.2067358E-732.47762351
ATCGTCA7150.032.32456610
CCGCATC1500.032.19499243
GCTCGTC8650.030.70620339
CTGGTCG8550.030.67331150
AGCGATA450.00462621530.6627718
GTACGCA1600.030.18366811
TCAAGAC2300.030.0022053
GTCAGCG8650.029.9086443
Produced by FastQC (version 0.11.5)