FastQCFastQC Report
Mon 6 Jun 2016
ERR1391390.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391390.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994954
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG608.221274E-626.23715820
GATCGGA651.5292675E-524.21891618
AGATCGG702.7138074E-522.48786517
ACCGCGC702.7138074E-522.4878659
CCGCGCC702.7138074E-522.48786510
AGCGGTT754.619216E-520.98972727
GCGGTTC807.587247E-519.6788628
GAGCGGT807.5901786E-519.6778726
ATCGGAA851.2087598E-418.52034819
ACGCCCG901.8730699E-417.4905612
CACCGCG901.8730699E-417.490568
GCCCGGC2251.8189894E-1115.9913714
CGCCCGG1552.0779044E-715.95912713
CGGTTCA1004.1802743E-415.74308829
CGGAAGA1004.181873E-415.74229621
ACCTCCG1056.060609E-414.99190935
CTTCGTA1209.794996E-514.9919087
CGTGAGC2451.0404619E-913.8215631
AGAGCGG1150.00120602513.68895325
TGCCCGG1150.001206482613.68826613
Produced by FastQC (version 0.11.5)