FastQCFastQC Report
Mon 6 Jun 2016
ERR1391389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745460
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG250.0020680736.1357574
ATCGCGC250.0020680736.1357575
ACCGCGC300.005161365529.9818449
CTGTACG450.001230484524.9848728
GATCGGA550.003946011420.44491218
AGATCGG707.008811E-419.27404217
GCGGTTC750.001114242217.98910728
CGCCACC1204.9862465E-616.9386375
GGGCGAC952.8307267E-416.56891428
GAGCGGT850.002574406615.87274326
GCGACAG1004.183812E-415.74046930
GGCGACA1156.812526E-515.64270229
AACGAAG1251.3865402E-414.3912878
CCCGCCA950.00524112814.2641153
ACCACGC2052.190609E-814.2596599
ACGAAGG1453.119208E-513.9570669
CAACGAA1801.1284101E-613.7416797
TCCAACG1656.6382363E-613.6877875
CGACAGA1150.001206585113.68736431
CCAACGA1709.034493E-613.2852056
Produced by FastQC (version 0.11.5)