FastQCFastQC Report
Mon 6 Jun 2016
ERR1391388.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391388.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064428
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGG250.002075109636.1121672
TCTCGTA401.5649384E-533.7358741
GACCGGC602.475131E-422.48952529
ATCTCGT654.2499398E-420.76053440
GAGACCG807.589444E-519.67833127
AGACCGG807.589444E-519.67833128
CCGAGAC851.2086422E-418.52078425
AAGAGCG750.001087211818.0560864
ATCTTCG1001.9923544E-518.0560845
CTCGTAT750.00111327317.99246642
CGGCCAA750.001113975117.99077417
GTGCCCG1002.0554487E-517.99077412
CGTATGC800.001715532616.86793544
ACCGGCT800.00171607216.86714230
ATGCCGA800.00171607216.86714222
CGGAAGA952.750025E-416.6329561
CGTATAA952.830154E-416.5704510
TTCACCG1104.5150795E-516.4146215
TCGTATA1004.1829608E-415.7419279
CGTGAGC2701.8189894E-1215.0488651
Produced by FastQC (version 0.11.5)