FastQCFastQC Report
Mon 6 Jun 2016
ERR1391387.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391387.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982467
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA801.0581425E-725.30090318
AGATCGG851.903336E-723.8101917
CGTATAA1052.3792381E-923.55943110
ATCGGAA903.3004653E-722.48969519
CCGAGAC955.5517376E-721.3071145
TCGTATA1401.382432E-1020.8822259
CTTCGTA1301.2350938E-920.758667
AGCGCTC707.0064404E-419.27597
AGCGGTT1202.5104782E-718.73950427
TCTTCGT1454.5802153E-918.6815746
ATCTTCG1506.9740054E-918.0588535
TTCGTAT1551.0963049E-817.410498
GCGGTTC1205.1966763E-616.86641128
TCGGAAG1257.657149E-616.1925820
GAGCGGT1301.1119499E-515.56820326
ACGGGAT1351.5894817E-514.99160313
AGAGCGG1351.5894817E-514.99160325
CGGTTCA1402.240049E-514.45692429
CGTTGGA1801.0812655E-613.7949585
ACTCCGT1000.007572903813.49381643
Produced by FastQC (version 0.11.5)