FastQCFastQC Report
Mon 6 Jun 2016
ERR1391383.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391383.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836470
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTA901.1974407E-824.98532116
ATGCCCG450.001230481124.98532112
CGGCTAA1003.6641723E-822.4867917
TCGTATA802.9883468E-622.4867889
CTTCGTA1009.100022E-720.2381117
TTAGCCG707.0089404E-419.2743926
TTCGTAT1002.0559131E-517.9894318
CCCGGCT1305.813581E-717.29753115
CGTATAA1053.126503E-517.13279210
TCTTCGT1351.5266509E-515.0550886
ATCTTCG1351.5266509E-515.0550885
CGTTGGA1604.8351358E-614.1141455
CCTTCGT1452.9953555E-514.0176491
TCGTTGG1656.647304E-613.6864444
CTTCGTT1555.6098295E-513.1124962
GCGTGGT1555.6098295E-513.1124964
AACCCGG1250.002259564112.59335422
TTCGTTG1801.6294034E-512.5459073
CCGCCAC1300.002938486212.1598794
AAACGGG1852.2547641E-512.15502211
Produced by FastQC (version 0.11.5)