FastQCFastQC Report
Mon 6 Jun 2016
ERR1391382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656646
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA600.006525228318.74121318
GCCACCG1402.1640686E-514.5083276
TCGTTGG1251.3438195E-414.4438464
CGTTGGA1301.8751381E-413.8883135
ACTAGAC1902.068331E-613.0202119
TTCGTTG1403.511443E-412.896293
AACGGGA1607.846528E-512.65031712
GGAAACG1801.6988039E-512.4941419
TCGCTTG1454.8627882E-412.40797415
CGGGATT1454.8627882E-412.40797414
GAAACGG1852.2502916E-512.15646210
CGTGAGC1701.3366288E-411.9489481
AAACGGG1902.9569577E-511.836555511
ACGGGAT1558.517262E-411.6074613
TGAGCCG1450.00684309710.85697745
TGCACTC2952.1377127E-710.67296115
GCACTCC2902.1634673E-59.30598116
GGGTGAC2000.00706343038.99578228
TGAACCC3301.0238227E-58.85948120
ACCATGC2801.50368E-48.8351429
Produced by FastQC (version 0.11.5)