FastQCFastQC Report
Mon 6 Jun 2016
ERR1391378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1391378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004574
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACGC300.0051582729.98652619
CGCAATT406.185416E-428.10957132
CCGCAAT406.185416E-428.10957131
GATCGGA802.9857547E-622.48989518
GCTCGAA550.003948728620.44332317
AGATCGG908.288762E-619.98902917
GCCACCG1302.665547E-819.1031326
AGCGCTC1302.7886927E-819.0280157
GCGCTCC1302.7886927E-819.0280158
ATCGGAA901.8717996E-417.49214219
TCGGAAG952.8285853E-416.57150320
GAATCGC1257.6640845E-616.19111312
AAGCGCT1551.9871368E-716.0219826
CGTATAA850.002574269715.87364110
CGTTGGA1855.3696567E-915.8645175
AGCGGTT1004.1822335E-415.74214327
ATCTTCG1156.587461E-515.7053425
CGGGATT1451.8185328E-615.50872914
CGCTCCA1602.9803778E-715.4602649
AACGGGA1754.8936272E-815.42010712
Produced by FastQC (version 0.11.5)